Research assistance

Members

The “pole biotechnologique” brings together a range of bioengineering expertise for the development of tools and approaches for research in evolutionary biology; and operational competences to ensure their logistical and technical implementation.

Since it was set up, the biotechnology hub has perpetuated insect breeding activities, including several species of drosophila, but also Venturia and Bemisia for example. These breeding activities are carried out on artificial medium or plants. We ensure laboratory logistics (e.g. autoclave, stock management, ...) as well as safety aspects, and develop devices (e.g. via 3D printing) to feed experimental research in the laboratory focusing on evolutionary genomics, behavioural ecology and functional ecology.

Our expertise is also solicited to organize field work, in metropolitan France and internationally, and to carry out population monitoring of terrestrial mammals (e.g. roe deer, marmots, feral cats, bats) and birds (dipper). In addition to capture (e.g. trapping, netting), marking and monitoring of animals, we implement experimental approaches in natura, collect biometric, behavioral and physiological data, biological samples (blood, secretions, hair, faeces, biopsy, ...) and characterize the study environments (installation of sensors, aerial imaging by drones, construction of digital models of terrain ...).

Finally, the biotechnology hub offers the technical resources and skills to analyse a wide variety of biological samples in the laboratory through an multidisciplinary prism. The complementarity and synergy of our competences are expressed in the fields of molecular biology (PCR, qPCR, construction of libraries for the genesis of ohmic data), cell biology (confocal microscopy, flow cytometry), and biochemistry (characterization of metabolic, endocrine and oxidative profiles).

Rehabilitation work was done at the LBBE in 2022 and lead to a significant expansion of the experimental space. These new premises allow us to increase our capacity, to offer new spaces and modern tools dedicated to behavioral approaches. These new premises also allow us to widen the spectrum of our activities by developing new techniques (e.g. cell culture, Crispr/cas9, RNAi, ...).

In all our activities, we regularly welcome and supervise trainees and students of all levels. For any questions or information request, do not hesitate to contact us

Publications

Display of 211 to 240 publications on 240 in total

Fat intake reverses the beneficial effects of low caloric intake on skeletal muscle mitochondrial H(2)O(2) production

B. Garait, K. Couturier, S. Servais, D. Letexier, D. Perrin, C. Batandier...

Free Radical Biology and Medicine . 39 : 1249-1261

DOI: 10.1016/j.freeradbiomed.2005.06.026

Journal article

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Assessment of forage availability in ecological studies

Sonia Saïd, Maryline Pellerin, Hervé Fritz, F. Débias, Hervé Fritz

European Journal of Wildlife Research . 51 ( 4 ) : 242-247

DOI: 10.1007/s10344-005-0115-y

Journal article

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Multiple infections and diversity of cytoplasmic incompatibility in a haplodiploid species

L. Mouton, H. Henri, M. Boulétreau, F. Vavre

Heredity . 94 : 187-192

Journal article

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Fat intake reverses the beneficial effect of low caloric intake on skeletal muscle mitochondrial H2O2 production

B. Garait, K. Couturier, S. Servais, D. Letexier, D. Perrin, C. Batandier...

Free Radical Biology and Medicine . 39 : 1249-1261

Journal article

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Origin and neofunctionalization of a Drosophila paternal effect gene essential for zygote viability.

Benjamin Loppin, David Lepetit, Steve Dorus, Pierre Couble, Timothy L Karr

Current Biology - CB . 15 ( 2 ) : 87-93

DOI: 10.1016/j.cub.2004.12.071

Journal article

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Uncoupling protein and ATP/ADP carrier increase mitochondrial proton conductance after cold adaptation of king penguin

D.A. Talbot, C. Duchamp, B. Rey, N. Hanuise, J.-L. Rouanet, B. Sibille...

The Journal of Physiology . 558 : 123-135

Journal article

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Mitochondrial proton conductance in cold adapted king penguins

C. Duchamp, D. Talbot, B. Rey, N. Hanuise, J.-L. Rouanet, B. Sibille...

Physiology News . 1 : 17-18

Journal article

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Virulence multiple infections and regulation of symbiotic population in the Wolbachia-Asobara tabida symbiosis

L. Mouton, F. Dedeine, H. Henri, M. Boulétreau, N. Profizi, F. Vavre

Genetics . 168 : 181-189

Journal article

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Uncoupling protein and ATP/ADP carrier increase mitochondrial proton conductance after cold adaptation of king penguins

D.A. Talbot, C. Duchamp, B. Rey, N. Hanuise, J.L. Rouanet, B. Sibille...

The Journal of Physiology . 558 : 123-135

DOI: 10.1113/jphysiol.2004.063768

Journal article

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Diversity distribution and specificity of WO phage infection in Wolbachia of four insect species

L. Gavotte, F. Vavre, H. Henri, M. Ravallec, R. Stouthamer, M. Boulétreau

Insect Molecular Biology . 13 : 147-153

Journal article

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The risk of hospital-acquired GB virus C infection: a pilot case-control study

Philippe Vanhems, N. Voirin, C. Trepo, M.A. Trabaud, D. Yzebe, C. del Signore...

Journal of Hospital Infection . 53 : 72-75

Journal article

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Strain-specific regulation of intracellular Wolbachia density in multiply infected insects

L. Mouton, H. Henri, M. Boulétreau, F. Vavre

Molecular Ecology . 12 : 3459-3465

Journal article

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Superoxide activates a GDP sensitive proton conductance in skeletal muscle mitochondria from king

D.A. Talbot, N. Hanuise, B. Rey, J.-L. Rouanet, C. Duchamp, M.D. Brand

Biochemical and Biophysical Research Communications . 312 : 983-988

Journal article

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WORLDWIDE DISTRIBUTION OF TRANSPOSABLE ELEMENT COPY NUMBER IN NATURAL POPULATIONS OF DROSOPHILA SIMULANS

Christian Biémont, Christiane Nardon, Grégory Deceliere, David Lepetit, Catherine Lœvenbruck, Cristina Vieira

Evolution - International Journal of Organic Evolution . 57 ( 1 ) : 159-167

DOI: 10.1111/j.0014-3820.2003.tb00225.x

Journal article

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Evolution of genome size in Drosophila. is the invader`s genome being invaded by transposable elements?

Cristina Vieira, C. Nardon, C. Arpin, D. Lepetit, C. Biémont

Molecular Biology and Evolution . 19 : 1154-1161

Journal article

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Insertion polymorphism of retrotransposable elements in populations of the insular endemic species Drosophila madeirensis

D. Lepetit, A. Brehm, P. Fouillet, C. Biémont

Molecular Ecology . 11 : 347-354

Journal article

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The diet of feral cats (Felis catus L.) at five sites on the Grande Terre, Kerguelen archipelago

Dominique Pontier, Ludovic Say, Francois Débias, Joël Bried, Jean Thioulouse, Hervé Fritz...

Polar Biology . 25 : 833-837

DOI: 10.1007/s00300-002-0424-5

Journal article

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Glider and Vision: two new families of miniature inverted-repeat transposable elements in Xenopus laevis genome

D. Lepetit, S. Pasquet, M. Olive, N. Thézé, P. Thiébaud

Genetica . 108 : 163-169

Journal article

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Transposable elements and genome evolution: the case of Drosophila simulans

Christian Biémont, Cristina Vieira, Nathalie Borie, David Lepetit

Genetica . 107 : 113-120

DOI: 10.1023/A:1003937603230

Journal article

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Phylogenetic evidence for horizontal transmission of Wolbachia in host-parasitoid associations

F. Vavre, Frédéric Fleury, D. Lepetit, P. Fouillet, M. Bouletreau

Molecular Biology and Evolution . 16 : 1711-1723

Journal article

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A novel tropomyosin isoform encoded by the Xenopus laevis α-TM gene is expressed in the brain

Catherine Gaillard, Nadine Thézé, Hubert Lerivray, Serge Hardy, David Lepetit, Pierre Thiébaud

Gene . 207 ( 2 ) : 235-239

DOI: 10.1016/s0378-1119(97)00634-3

Journal article

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A temperature cline in copy number for 412 but not roo/B104 retrotransposons in populations of Drosophila simulans

Cristina Vieira, P. Aubry, D. Lepetit, C. Biémont

Proceedings of the Royal Society B: Biological Sciences . 265 ( 1402 ) : 1161-1165

DOI: 10.1098/rspb.1998.0413

Journal article

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Inhibition of sex pheromone communications of Trichogramma Brassicae (Hymenoptera) by the insecticide chlorpyrifos

Jean-Marie Delpuech, B. Froment, P. Fouillet, F. Pompanon, S. Janillon, P. Boulétreau

Environmental Toxicology and Chemistry . 17(6) : 1107-1113

Journal article

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The distribution of minisatellites in the human, pig and rat genome reflects the history of rearrangements involving chromosome-ends

Valérie Amarger, D. Gauguier, Martine M. Yerle, S. Monfouilloux, F. Giraudeau, Philippe Pinton...

Animal Genetics . suppl. 2 : 75

Journal article

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The distribution of minisatellites in the human, pig and rat genome reflects the history of rearrangements involving chromosome-ends

Valérie Amarger, D. Gauguier, Martine M. Yerle, S. Monfouilloux, F. Giraudeau, Philippe Pinton...

XXV International Conference on Animal Genetics, International Society for Animal Genetics .

Conference paper

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The cloning and characterization of a cDNA encoding Xenopus laevis DNA ligase I.

David Lepetit, Pierre Thiebaud, Saïd Aoufouchi, Claude Prigent, Rozène Guesné, Nadine Thézé

Gene . 172 ( 2 ) : 273-7

Journal article

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The Xenopus laevis TM-4 gene encodes non-muscle and cardiac tropomyosin isoforms through alternative splicing

Serge Hardy, Nadine Thézé, David Lepetit, Marie-Rose Allo, Pierre Thiebaud

Gene . 156 ( 2 ) : 265-270

DOI: 10.1016/0378-1119(94)00929-m

Journal article

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Detection, cloning, and distribution of minisatellites in some mammalian genomes

G. Vergnaud, D. Gauguier, J.-J. Schott, D. Lepetit, V. Lauthier, D. Mariat...

DNA Fingerprinting: State of the Science . : 47-57

DOI: 10.1007/978-3-0348-8583-6_4

Book chapter

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Detection, cloning, and distribution of minisatellites in some mammalian genomes.

Gilles Vergnaud, D Gauguier, J J Schott, D Lepetit, V Lauthier, D Mariat...

EXS . 67 : 47-57

DOI: 10.1007/978-3-0348-8583-6_4

Journal article

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