GECO Evolutionary and Computational Genomics

Bioinformatics, Phylogeny and Evolutionary Genomics Group

Necsulea Anamaria

Chargée de recherche

CNRS

Address: 43 bd du 11 novembre 1918
69622 VILLEURBANNE cedex

Tel: 04 72 43 35 82

Email

A primary goal in biology is to construct a functional map of the genome, linking each DNA sequence to its molecular roles and to the cellular and organismal processes in which it takes part. Thanks to the development of molecular technologies, which provide genome-wide maps of transcription, protein and RNA binding, chromatin state and topology, etc., we are now considerably closer to this goal. However, uncovering the biochemical activity associated with each DNA sequence is not sufficient to prove its functionality, as numerous active genomic elements may be redundant or altogether dispensable, resulting from inherent noise in cellular processes. Evolutionary genomics approaches can help bridge the gap between biochemical activity and biological functionality, by identifying genomic elements that are subject to purifying natural selection to maintain their functional properties or to positive selection following the acquisition of new functions. With this motivation, I am interested in the evolution of long non-coding RNAs, a class of transcripts that are pervasive in mammalian genomes and whose functionality is still largely debated (Necsulea et al., 2014; Darbellay & Necsulea, 2020).  I have addressed this question through comparative transcriptomics analyses across multiple species, tissues and developmental stages. 

Another facet of my research focuses on the evolution of gene expression regulatory mechanisms. I am particularly interested in the organization of mammalian cis-regulatory landscapes, in particular in the long-range interactions between gene promoters and distant regulatory elements.  Data on long-range contacts between promoters and regulatory elements is rapidly accumulating. However, it remains unclear how these regulatory relationships evolve and how they contribute to the establishment of robust gene expression profiles. Alexandre Laverré, currently a PhD student jointly supervised with Eric Tannier, addresses this question by exploiting high-throughput chromatin conformation capture data for human and mouse (Laverré, Tannier & Necsulea, in revision). 

 

Publications

Display of 1 to 30 publications on 30 in total

Evolution and functional relevance of non-coding elements in vertebrate genomes

Anamaria Necsulea

HDR

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Meta-analysis reveals limited reproducibility of lncRNA-related findings in hepatocellular carcinoma research

Anamaria Necsulea, Philippe Veber, Tuyana Boldanova, Charlotte Ky Ng, Stefan Wieland, Markus H Heim

DOI: 10.1101/2024.08.17.608405

Preprint

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GTDrift: a resource for exploring the interplay between genetic drift, genomic and transcriptomic characteristics in eukaryotes

Florian Bénitière, Laurent Duret, Anamaria Necsulea

NAR Genomics and Bioinformatics . 6 ( 2 ) : lqae064

DOI: 10.1093/nargab/lqae064

Journal article

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LncRNA analyses reveal increased levels of non-coding centromeric transcripts in hepatocellular carcinoma

Anamaria Necsulea, Philippe Veber, Tuyana Boldanova, Charlotte K Y Ng, Stefan Wieland, Markus H Heim

DOI: 10.1101/2021.03.03.433778

Preprint

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Random genetic drift sets an upper limit on mRNA splicing accuracy in metazoans

Florian Bénitière, Anamaria Necsulea, Laurent Duret

eLife . 13 : RP93629

DOI: 10.7554/eLife.93629.3

Journal article

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GOntact: using chromatin contacts to infer Gene Ontology enrichments for cis -regulatory elements

Alexandre Laverré, Eric Tannier, Philippe Veber, Anamaria Necsulea

DOI: 10.1101/2022.06.13.495495

Preprint

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Long-range promoter–enhancer contacts are conserved during evolution and contribute to gene expression robustness

Alexandre Laverré, Eric Tannier, Anamaria Necsulea

Genome Research . 32 ( 2 ) : 280-296

DOI: 10.1101/gr.275901.121

Journal article

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Phylogenomics and Genome Annotation

Anamaria Necsulea

Phylogenetics in the Genomic Era . : 4.1:1--4.1:26

Book chapter

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Comparative Transcriptomics Analyses across Species, Organs, and Developmental Stages Reveal Functionally Constrained lncRNAs

Fabrice Darbellay, Anamaria Necsulea

Molecular Biology and Evolution .

DOI: 10.1093/molbev/msz212

Journal article

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Transcriptional response to hepatitis C virus infection and interferon-alpha treatment in the human liver

T. Boldanova, A. Suslov, M.H. Heim, A. Necsulea

EMBO Molecular Medicine . 9 : 816-834

DOI: 10.15252/emmm.201607006

Journal article

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The fitness cost of mis-splicing is the main determinant of alternative splicing patterns

Baptiste Saudemont, Alexandra Popa, Joanna Parmley, Vincent Rocher, Corinne Blugeon, Anamaria Necsulea...

Genome Biology . 18 : 208

DOI: 10.1186/s13059-017-1344-6

Journal article

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Control of Hoxd gene transcription in the mammary bud by hijacking a preexisting regulatory landscape

R. Schep, A. Necsulea, E. Rodriguez-Carballo, I. Guerreiro, G. Andrey, T.H. Nguyen Huynh...

Proceedings of the National Academy of Sciences of the United States of America . 113 ( 48 ) : e7720-e7729

DOI: 10.1073/pnas.1617141113

Journal article

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A role for HOX13 proteins in the regulatory switch between TADs at the HoxD locus

L. Beccari, N. Yakushiji-Kaminatsui, J. M. Woltering, A. Necsulea, N. Lonfat, E. Rodriguez-Carballo...

Genes and Development . 30 : 1172-86

DOI: 10.1101/gad.281055.116

Journal article

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Assessing Recent Selection and Functionality at Long Noncoding RNA Loci in the Mouse Genome

R. A. Wiberg, D. L. Halligan, R. W. Ness, A. Necsulea, H. Kaessmann, P. D. Keightley

Genome Biology and Evolution . 7 : 2432-44

DOI: 10.1093/gbe/evv155

Journal article

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Cellular Source and Mechanisms of High Transcriptome Complexity in the Mammalian Testis

M. Soumillon, A. Necsulea, M. Weier, D. Brawand, X. Zhang, H. Gu...

Cell Reports . 3 ( 6 ) : 2179-2190

DOI: 10.1016/j.celrep.2013.05.031

Journal article

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Mechanisms and Evolutionary Patterns of Mammalian and Avian Dosage Compensation

Philippe Julien, David Brawand, Magali Soumillon, Anamaria Necsulea, Angélica Liechti, Frédéric Schütz...

PLoS Biology . 10 ( 5 ) : e1001328

DOI: 10.1371/journal.pbio.1001328

Journal article

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The evolution of gene expression levels in mammalian organs

David Brawand, Magali Soumillon, Anamaria Necsulea, Philippe Julien, Gábor Csárdi, Patrick Harrigan...

Nature . 478 ( 7369 ) : 343-348

DOI: 10.1038/nature10532

Journal article

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Meiotic recombination favors the spreading of deleterious mutations in human populations

Anamaria Necsulea, L. Duret, Alexandra Popa, David N. Cooper, Peter Stentson, Dominique Mouchiroud...

Human Mutation . 32 ( 2 ) : 198-206

DOI: 10.1002/humu.21407

Journal article

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Complete genome of the cellulolytic thermophile Acidothermus cellulolyticus 11B provides insights into its ecophysiological and evolutionary adaptations

Ravi D. Barabote, Gary Xie, David H. Leu, Philippe Normand, Anamaria Necsulea, Vincent Daubin...

Genome Research . ( 19 ) : 1033-1043

DOI: 10.1101/gr.084848.108

Journal article

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Monoallelic expression and tissue specificity are associated with high crossover rates

A. Necşulea, M. Sémon, L. Duret, L.D. Hurst

Trends in Genetics . 25(12) : 519-522

Journal article

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The relationship between DNA replication and human genome organization.

Anamaria Necsulea, Claire Guillet, Jean-Charles Cadoret, Marie-Noëlle Prioleau, L. Duret

Molecular Biology and Evolution . 26 ( 4 ) : 729-741

DOI: 10.1093/molbev/msn303

Journal article

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Etude des patrons d`évolution asymétrique dans les séquences d`ADN

A. Necşulea

incollection . -- : 476-482

Journal article

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Etude des patrons d'évolution asymétrique dans les séquences d'ADN

Anamaria Necsulea

Thesis

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Parallel Adaptations to High Temperatures in the Archean Eon

Bastien Boussau, Samuel Blanquart, Anamaria Necsulea, Nicolas Lartillot, Manolo Gouy

Nature . 456 ( 7224 ) : 942-945

DOI: 10.1038/nature07393

Journal article

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A New Method for Assessing the Effect of Replication on DNA Base Composition Asymmetry

A. Necşulea, J.R. Lobry

Molecular Biology and Evolution . 24 : 2169-2179

Journal article

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A New Method for Assessing the Effect of Replication on DNA Base Composition Asymmetry

A. Necşulea, J.R. Lobry

incollection . 7 : 379-381

Journal article

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Synonymous codon usage and its potential link with optimal growth temperature in prokaryotes.

J.R. Lobry, A. Necşulea

Gene . 385 : 128-136

Journal article

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Revisiting the directionnal mutation pressure theory : The analysis of a particular genomic structure in Leishmania major

A. Necşulea, J.R. Lobry

Gene . 385 : 28-40

Journal article

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